自我介紹/ 經驗 Self-Introduction/ Past experiences :
嗨！我叫Ananda Gabo（https://www.instagram.com/a__gabo/），我是加拿大多倫多的一名工業，藝術家和合同研究人員。在過去的十年中，我一直在DIY生物學領域（具有不同的複雜性水平）中使用廉價的非常規材料來探索低成本的製造方法，同時還經常在覓食和做飯。這裡沒有任何生物學小組（儘管我現在已經在設法組織一個小組，因為我擁有技能和資源），但是過去十年來我一直在學習和旅行中學習各種DIY生物學技能和黑客文化。

在業餘時間（和自有資金，因為沒有這種編程的支持），我也在運行Paocai Bio。一個講習班系列，通過東亞流散（遺產）鏡頭研究基於社區的生物學。參加研討會的大多數人是東亞科學專業人士（他們想做更多的藝術）或沒有生物學經驗的藝術家。到目前為止，我只是專注於食品+發酵，中藥和瓊脂藝術，但我希望最終讓我的參與者更多地從事DIY科學硬件或編輯自己的生物的工作。

Hi! My name is Ananda Gabo (https://www.instagram.com/a__gabo/) and I am an industrial, artist and contract researcher based in Toronto, Canada. Over the last 10 years, I've been using craft and unusual materials to explore low-cost fabrication methods in the realm of D-I-Y biology (in varying levels of complexity) as well as being a forager-in-training and cook. There aren't any groups for biology here (although I am trying to organize one here now that I have the skills and resources) but I've spent the last 10 years learning and traveling where I can to learn about various skills in DIY biology and hacker culture.

In my spare time (and own funds as there is no support for this kind of programming) I also am running Paocai Bio. A workshop series that looks at community based biology through an east Asian diasporic (heritage) lens. Most of the workshop attendees are either east Asian science professionals (who want to do more art) or artists who don't have any experience with biology. So far I have just managed to focus on food + fermentation, traditional Chinese medicine and agar art, but I would love to eventually have my participants work more on DIY science hardware or editing their own organisms.

Website/ Portfolio 網站/ 作品集:
我現在沒有投資組合或網站，因為我仍在嘗試記錄和整理最近的工作。但是這裡是我過去從事過的一些手工藝品項目/一些插圖項目的鏈接。 它可能確實是過時的，並且不包括我所做的一切

I don't have a portfolio or website up right now because I am still trying to document and assemble my recent work. But here is a link to some craft projects/ some illustration projects I've worked on in the past. It might be really dated though and doesn't include everything I've done:

https://drive.google.com/file/d/1cZIt_QNj8jzgyQmAJWTv6rl5LdwsrfCG/view?usp=sharing

還有這裡是泡菜計劃（Paocai）的instagram
And here is a link to Paocai Bio:
https://www.instagram.com/paocaibio

想分享的技術或技藝或概念 Technique/Concept that I want to share :

低保真的紅茶菌文化Kombucha cultures for low-fidelity prototyping:
因此，我知道有很多人一直在使用紅茶菌作為製作材料。但我喜歡它，因為它非常易於訪問且具有彈性。我一直在探索如何將其種植到方便的容器中，而不是在方墊中種植它。我希望有一天能夠生長更大，更多的實驗性物品以及使用軟機器人進行實驗，我曾在2018年進行過實驗，將致動器和傳感器種植到康普茶生物膜中，但它確實很亂且文獻記載很少。探索成長“扁平包裝”成長型形狀，圖案和軟外殼的概念將是很棒的。

第1步：創建要生長的平面紙原型。細菌纖維素將在表面接觸氧氣的地方形成。因此，如果您有心形血管，就會有心形文化！

第2步：將3d打印，激光切割或將容器製成物體，並用中性矽酮密封任何裂縫。某些有機矽填縫劑具有防黴性，儘管我尚未在水箱中進行過測試，但為防止萬一，我只使用了水族館級有機矽。

第3步：添加封面，讓它成長！我還沒有弄清楚的是如何將執行器安全地嵌入其中。或者提出了一種測量紅茶菌文化萎縮的標準方法。但是我認為紅茶菌是一種很好的低保真原型材料，因為它具有較高的pH值和可用性。這是它的外觀示例：

最重要的是，我通常喜歡發送測序樣本，以了解我的培養物中存在哪些生物菌株。它完全沒有必要，但是我喜歡手工藝中的基因記錄的想法。

So I know that so many people have been using kombucha as a crafting material. But I love it as its extremely accessible and resilient. I've been exploring how to grow it into convenient vessels instead of growing it in square mats. I would love one day to grow larger and more experimental pieces as well as experimenting with soft robotics, I've experimented in 2018 growing actuators and sensors into Kombucha biofilm but it was really messy and poorly documented. It would be great to explore the concept of growing "flat packed" grow able shapes, patterns, and soft-enclosures.

Step 1: Create paper prototypes of flat surfaces that you would like to grow. Bacterial cellulose will form where the surface touches oxygen. So if you have a heart shaped vessel, you'll have a heart shaped culture!

Step 2: 3d print, laser cut, or fabricate vessles into the objects and seal any cracks with neutral silicone. Some silicone caulking has anti molding in it and although I haven't tested it with my tanks, I just use aquarium grade silicone just in case.

Step 3: Add a cover and let it grow! Some things I haven't figured out yet are how to securely embed actuators into it. Or have come up with a standard way of measuring shrinkage of the kombucha cultures. But I think kombucha is a great low-fidelity prototyping material due to its high pH and its availability. Here is an example of what it can look like:

On top of this, I usually like to send out samples of sequencing to know what strains of organisms exist within my culture. Its completely unnecessary, but I love the idea of genetic documentation in crafts.